https://data.archives-ouvertes.fr/document/hal-03216664v1

https://data.archives-ouvertes.fr/document/hal-03216664v1

http://purl.org/dc/terms/available 2021-06-18
http://purl.org/dc/terms/creator _:vb15695275
http://purl.org/dc/terms/type https://data.archives-ouvertes.fr/doctype/Article
http://purl.org/dc/terms/bibliographicCitation Camille Henry, Laurent Loiseau, Alexandra Vergnes, Didier Vertommen, Angela Mérida-Floriano, et al.. Redox controls RecA protein activity via reversible oxidation of its methionine residues. eLife, 2021, 10, ⟨10.7554/eLife.63747⟩. ⟨hal-03216664⟩
http://purl.org/dc/elements/1.1/language en
http://purl.org/dc/terms/identifier hal-03216664
http://www.openarchives.org/ore/terms/aggregates https://amu.hal.science/hal-03216664v1/file/elife-63747-v3.pdf
http://purl.org/dc/terms/abstract Reactive oxygen species (ROS) cause damage to DNA and proteins. Here, we report that the RecA recombinase is itself oxidized by ROS. Genetic and biochemical analyses revealed that oxidation of RecA altered its DNA repair and DNA recombination activities. Mass spectrometry analysis showed that exposure to ROS converted four out of nine Met residues of RecA to methionine sulfoxide. Mimicking oxidation of Met35 by changing it for Gln caused complete loss of function, whereas mimicking oxidation of Met164 resulted in constitutive SOS activation and loss of recombination activity. Yet, all ROS-induced alterations of RecA activity were suppressed by methionine sulfoxide reductases MsrA and MsrB. These findings indicate that under oxidative stress MsrA/B is needed for RecA homeostasis control. The implication is that, besides damaging DNA structure directly, ROS prevent repair of DNA damage by hampering RecA activity.
http://data.archives-ouvertes.fr/schema/pubmedcentral PMC7943192
http://purl.org/ontology/bibo/doi 10.7554/eLife.63747
http://purl.org/dc/terms/issued 2021-02-19
http://purl.org/dc/terms/isVersionOf https://data.archives-ouvertes.fr/document/hal-03216664
http://purl.org/dc/terms/language http://lexvo.org/id/iso639-1/en
http://purl.org/dc/terms/identifier https://amu.hal.science/hal-03216664
http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://purl.org/spar/fabio/Article
http://purl.org/ontology/bibo/volume 10
http://purl.org/dc/terms/title Redox controls RecA protein activity via reversible oxidation of its methionine residues
http://purl.org/dc/terms/source https://data.archives-ouvertes.fr/anrProject/44161
http://purl.org/dc/terms/rights http://creativecommons.org/licenses/by/
http://purl.org/dc/terms/isPartOf https://amu.hal.science/ANR
http://purl.org/dc/terms/isPartOf https://amu.hal.science/PASTEUR
http://data.archives-ouvertes.fr/schema/pubmed 33605213
http://www.w3.org/2002/07/owl#sameAs https://dx.doi.org/10.7554/eLife.63747
http://www.w3.org/2002/07/owl#sameAs https://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=33605213
http://purl.org/dc/terms/isPartOf https://amu.hal.science/CNRS
http://purl.org/dc/terms/isPartOf https://data.archives-ouvertes.fr/revue/90086
http://purl.org/dc/terms/creator _:vb15695268
http://purl.org/dc/terms/creator _:vb15695269
http://www.w3.org/2002/07/owl#sameAs https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7943192
http://purl.org/dc/terms/creator _:vb15695270
http://purl.org/dc/terms/isPartOf https://amu.hal.science/UNIV-AMU
http://purl.org/dc/terms/contributor _:vb15695265
http://purl.org/dc/terms/isPartOf https://amu.hal.science
http://purl.org/dc/terms/isPartOf https://amu.hal.science/FRM
http://purl.org/dc/terms/creator _:vb15695271
http://purl.org/dc/terms/creator _:vb15695266
http://purl.org/dc/terms/creator _:vb15695267
http://purl.org/dc/terms/creator _:vb15695276
http://data.archives-ouvertes.fr/schema/topic https://data.archives-ouvertes.fr/subject/sdv
http://purl.org/dc/terms/created 2021-06-18T16:26:24
http://purl.org/dc/terms/creator _:vb15695272
http://purl.org/dc/terms/modified 2025-09-30T03:16:19
http://purl.org/dc/terms/creator _:vb15695273
http://purl.org/dc/terms/creator _:vb15695274

_:vb15695270

http://data.archives-ouvertes.fr/schema/structure https://data.archives-ouvertes.fr/structure/254694
http://xmlns.com/foaf/0.1/name Angela Mérida-Floriano
http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://data.archives-ouvertes.fr/schema/Author

_:vb15695272

http://xmlns.com/foaf/0.1/name Elizabeth Wood
http://data.archives-ouvertes.fr/schema/structure https://data.archives-ouvertes.fr/structure/44110
http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://data.archives-ouvertes.fr/schema/Author

_:vb15695267

http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://data.archives-ouvertes.fr/schema/Author
http://data.archives-ouvertes.fr/schema/structure https://data.archives-ouvertes.fr/structure/479
http://xmlns.com/foaf/0.1/name Laurent Loiseau

_:vb15695266

http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://data.archives-ouvertes.fr/schema/Author
http://data.archives-ouvertes.fr/schema/structure https://data.archives-ouvertes.fr/structure/479
http://xmlns.com/foaf/0.1/name Camille Henry
http://data.archives-ouvertes.fr/schema/structure https://data.archives-ouvertes.fr/structure/44110

_:vb15695276

http://data.archives-ouvertes.fr/schema/structure https://data.archives-ouvertes.fr/structure/479
http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://data.archives-ouvertes.fr/schema/Author
http://data.archives-ouvertes.fr/schema/person https://data.archives-ouvertes.fr/author/750788

_:vb15695273

http://xmlns.com/foaf/0.1/name Josep Casadesús
http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://data.archives-ouvertes.fr/schema/Author
http://data.archives-ouvertes.fr/schema/structure https://data.archives-ouvertes.fr/structure/254694

_:vb15695268

http://xmlns.com/foaf/0.1/name Alexandra Vergnes
http://data.archives-ouvertes.fr/schema/structure https://data.archives-ouvertes.fr/structure/479
http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://data.archives-ouvertes.fr/schema/Author

_:vb15695275

http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://data.archives-ouvertes.fr/schema/CorrespondingAuthor
http://data.archives-ouvertes.fr/schema/structure https://data.archives-ouvertes.fr/structure/1008146
http://data.archives-ouvertes.fr/schema/structure https://data.archives-ouvertes.fr/structure/479
http://data.archives-ouvertes.fr/schema/person https://data.archives-ouvertes.fr/author/1167435

_:vb15695274

http://data.archives-ouvertes.fr/schema/structure https://data.archives-ouvertes.fr/structure/44110
http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://data.archives-ouvertes.fr/schema/Author
http://xmlns.com/foaf/0.1/name Michael Cox

_:vb15695269

http://data.archives-ouvertes.fr/schema/structure https://data.archives-ouvertes.fr/structure/92863
http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://data.archives-ouvertes.fr/schema/Author
http://data.archives-ouvertes.fr/schema/person https://data.archives-ouvertes.fr/author/761289

_:vb15695271

http://data.archives-ouvertes.fr/schema/structure https://data.archives-ouvertes.fr/structure/44110
http://xmlns.com/foaf/0.1/name Sindhu Chitteni-Pattu
http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://data.archives-ouvertes.fr/schema/Author

https://data.archives-ouvertes.fr/document/hal-03216664

http://purl.org/dc/terms/hasVersion https://data.archives-ouvertes.fr/document/hal-03216664v1

Télécharger au format